An Adenosine 3 ’ , 5 ’ - Monophosphate - de - pendent
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چکیده
In order to provide further information regarding one of the NHzOH-sensitive bonds, the appropriate peptides were subjected to Edman degradation (16) and the phenylthiohydantoin amino acids identified by thin layer chromatography on sheets of silica gel containing a fluorescent indicator (Eastman). Chromatograms were developed with Solvents D and E of Edman and Sjijquist (17). The results of amino acid analyses of residual peptides, performed after each degradation step of T4, agreed with those obtained by thin layer chromatography of the phenylthiohydantoin amino acids. These experiments indicated the following partial sequences:
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تاریخ انتشار 2003